Job Description

Job Description

We are seeking a highly skilled, passionate, and motivated scientist to join our diabetes research team. This position will play a key lab-based role in advancing iPSC stem cell engineering and contribute to the development of the next generation of islet cell therapies.

Responsibilities:

• Enable and advance iPSC gene editing, cell line development, and evaluation of differentiated cells.
• Perform routine iPSC cell culture, passaging, and media preparation.
• Optimize workflows for iPSC CRISPR-based gene editing, gRNA screening, and delivery.
• Develop and perform assays for on-/off-target analysis using PCR and NGS workflows.
• Generate clonal gene-edited iPSC lines and characterize them via flow cytometry, ddPCR, dPCR, and qPCR.
• Culture and execute routine directed differentiation of human iPSCs to pancreatic islets, performing assessments such as cell counts, viability, and metabolite analysis.
• Assist scientists in evaluating and designing mitigation strategies to ensure genomic stability of iPSC lines and drug substances.
• Collaborate cross-functionally to support the transfer of R&D assays to GMP environments.
• Maintain accurate and detailed experimental records, synthesize results, prepare reports, and present findings in meetings.
• Contribute to the development and refinement of SOPs.
• Execute work plans efficiently and meet established deadlines.

Qualifications:

• BS or MS in Stem Cell Biology, Cell Biology, Bioengineering, or a related field.
• 3+ years of relevant industry experience for MS candidates, or 5+ years for BS candidates.
• Extensive hands-on experience with human iPSC culture, gene editing, and molecular biology techniques.
• Proficiency in gene editing and cell line development, including protocol optimization and flow-based sorting.
• Hands-on experience with delivery methods for gene editing tools.
• Strong communication and interpersonal skills, with the ability to work effectively in a collaborative team environment.
• Excellent organizational skills and attention to detail in record keeping.
• Experience in pancreatic islet directed differentiation is highly desirable.
• Experience in transitioning research-grade processes to GMP-grade is highly desirable.

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